The Flat Electrode can be used for both electroporation and electro cell fusion. The Flat Electrode generates either a divergent or homogeneous field depending on the orientation of the grooved electrodes. The Flat Electrode can be oriented with the grooved sides of the electrode facing one another to generate a divergent field for use in electro cell fusion. Alternatively, it can be oriented with the flat sides facing each other providing a homogeneous field for electroporation. The Electrode is made of two rectangular, parallel plates of high grade stainless steel that are press-fitted into a polysulfone base.
USE OF THE FLAT ELECTRODE WITH THE ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM
In this example, the ECM 2001 generator is connected using the sequence of cables and adapters illustrated below, then connected to the flat electrode. This electrode can also be used with the ECM 830 and Gemini X2 systems.
|ECM 2001 System||45-0083 Coaxial Cables||45-0088 Female/Female Adapters||45-0217 Flat Electrode Cables|
|Generator Compatibility||ECM 830, ECM 2001 and Gemini X2|
|Field Type||Divergent or Homogeneous|
- Cell fusion
- Hybridoma production
- Plant protoplast fusion
- Mammalian cell transfection
|45-0217||ECM 2001 Banana to Banana Cables, Red and Black, 10 ft (for use with 45-0108 Flat Electrode)|
Long-Bo Cui et al. Transfer of germinal vesicle to ooplasm of young mice could not rescue ageing-associated chromosome misalignment in meiosis of oocytes from aged mice. Human Reproduction, Vol. 20, No.6 pp. 1624-1631 (2005).
Grabarek et al. Efficient Delivery of dsRNA into Zona-Enclosed Mouse Oocytes and Preimplantation Embryos by Electroporation. Genesis 32:269-276 (2002)