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ECM 830 Square Wave Electroporation System

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ECM 830 Square Wave Electroporation System
Versatile electroporation system suitable for applications for gene, drug and protein delivery including: mammalian cells, in vivo, ex vivo, in ovo tissue, ex plant, nuclear transfer, CRISPR
Product Item # Price  
ECM 830 Electroporation System Complete, for Cuvettes (N. America) 45-0002   View Price
ECM 830 Electroporation System Complete, for Cuvettes 45-0002INT   View Price
ECM 830 Generator Only (N. America) 45-0052   View Price
ECM 830 Generator Only 45-0052INT   View Price
ECM 830 High Throughput Electroporation System, 25-well with HT-100 (N. America) 45-0410   View Price
ECM 830 High Throughput Electroporation System, 25-well with HT-100 45-0410INT   View Price
ECM 830 High Throughput Electroporation System, 25-well with HT-200 (N. America) 45-0411   View Price
ECM 830 High Throughput Electroporation System, 25-well with HT-200 45-0411INT   View Price
ECM 830 High Throughput Electroporation System, 96-well with HT-100 (N. America) 45-0420   View Price
ECM 830 High Throughput Electroporation System, 96-well with HT-100 45-0420INT   View Price
ECM 830 High Throughput Electroporation System, 96-well with HT-200 (N. America) 45-0421   View Price
ECM 830 High Throughput Electroporation System, 96-well with HT-200 45-0421INT   View Price
ECM 830 Electroporation System Complete, for Cuvettes with Monitoring (N. America) 45-0072   View Price
ECM 830 Electroporation System Complete, for Cuvettes with Monitoring 45-0072INT   View Price
ECM 830 High Throughput Electroporation System with Monitoring (N. America) 45-0472   View Price
ECM 830 High Throughput Electroporation System with Monitoring 45-0472INT   View Price
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Details

ECM 830 GENERATOR

The ECM 830 is a square wave pulse generator designed for in vitro and in vivo applications. The versatility of the ECM 830 applications for gene, drug and protein delivery include; mammalian cells, in vivo, ex vivo tissue, zebra fish tissue and embryos, nuclear transfer, embryo manipulation, plant protoplast and basic bacteria and yeast transformations. All accessories plug directly into the unit.

The ECM 830 can be used in combination with a wide array of BTX specialty electrodes and accessories to enhance your molecular and drug delivery experiments.

FEATURES

  • A wide range of voltages from 5 to 3,000 V
  • Finer voltage discrimination
  • Pulse durations from 10 μsec to 10 sec
  • Arc Quenching
  • Digital display of actual voltage and pulse length delivered

IN VIVO APPLICATIONS

  • Mammalian cell transfection
  • In vitro, in vivo, ex vivo and in ovo tissue transfection
  • Nuclear transfer
  • Ex plant tissue transfection
  • Plant protoplast transfection
  • siRNA libraries
  • High throughput 25- and 96-well electroporation

ADHERENT CELL TRANSFECTION

The ECM 830 generator coupled with the Petri Pulser electrode or the adherent cell electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in. The Petri Pulser electrode is ideal for 6-well plates and the adherent cell electrodes for 12-well plates.

HIGH THROUGHPUT (HT) ELECTROPORATION (45-0411 and 45-0421)

The High Throughput (HT) 96- and 25-well systems offer the advantage of multi-well electroporation technology for processing multiple samples in seconds. 

This permits a large number of samples to be quickly electroporated using high throughput plate handler technology. Using the HT Multi-Well plates instead of traditional cuvettes, the researcher can transition from a single cuvette to either a 96-well or 25-well electroporation plate by using a plate handler. This increases yields and the number of experiment runs in a single day. Experiments take seconds to run, allowing for quick and efficient optimization of the electrical and biological parameters. Once optimized, samples are rapidly processed increasing yields and saving valuable time and money.

The key to the HT System is the combination of the ECM 830 Generator with the Plate Handler and multi-well plates. The Plate Handler holds the plates and delivers the pulse(s) to the wells using parameters set in the ECM 830 generator.

MONITORING OPTION

The ENHANCER 3000 allows the researcher to monitor and track key electrical parameters used in electroporation applications. The electrical pulse data is captured as both a graphic display of the wave form and electrical output values following each electroporation experiment. 

INCLUDED ITEMS

Item # Product Included Items
45-0002 ECM 830 Electroporation System Complete, for Cuvettes (N.America) ECM 830 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660
45-0002INT ECM 830 Electroporation System Complete, for Cuvettes ECM 830 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each) and Cuvette Rack 660
45-0052 ECM 830 Generator Only (N. America) ECM 830 Generator only
45-0052INT ECM 830 Generator Only ECM 830 Generator only
45-0010 ECM 830 High Throughput Electroporation System, 25-well with HT-100 (N. America) ECM 830 Generator, 25-Well Plates (2 mm gap, 6X), Plate Seals, HT-100 Plate Handler and a plate adaptor
45-0010INT ECM 830 High Throughput Electroporation System, 25-well with HT-100 ECM 830 Generator, 25-Well Plates (2 mm gap, 6X), Plate Seals, HT-100 Plate Handler and a plate adaptor
45-0011 ECM 830 High Throughput Electroporation System, 25-well with HT-200 (N. America) ECM 830 Generator, 25-Well Plates (2 mm gap, 6X), Plate Seals, HT-200 Plate Handler and a plate adaptor
45-0011INT ECM 830 High Throughput Electroporation System, 25-well with HT-200  ECM 830 Generator, 25-Well Plates (2 mm gap, 6X), Plate Seals, HT-200 Plate Handler and a plate adaptor
45-0420 ECM 830 High Throughput Electroporation System, 96-well with HT-100 (N. America) ECM 830 Generator, 96-Well Plates (2 mm gap, 2X), Plate Seals, HT-100 Plate Handler and a plate adaptor
45-0420INT ECM 830 High Throughput Electroporation System, 96-well with HT-100 ECM 830 Generator, 96-Well Plates (2 mm gap, 2X), Plate Seals, HT-100 Plate Handler and a plate adaptor
 45-0421 ECM 830 High Throughput Electroporation System, 96-well with HT-200 (N. America) ECM 830 Generator, 96-Well Plates (2 mm gap, 2X), Plate Seals, HT-200 Plate Handler and a plate adaptor
 45-0421INT ECM 830 High Throughput Electroporation System, 96-well with HT-200 ECM 830 Generator, 96-Well Plates (2 mm gap, 2X), Plate Seals, HT-200 Plate Handler and a plate adaptor
45-0072 ECM 830 Electroporation System Complete, for Cuvettes with Monitoring (N. America) ECM 830 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660, ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope and Cables
45-0072INT ECM 830 Electroporation System Complete, for Cuvettes with Monitoring ECM 830 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660, ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope and Cables
45-0472 ECM 830 High Throughput Electroporation System with Monitoring (N. America) ECM 830 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660, ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope, Cables, 25-Well Plates (2 mm gap, 6X), HT-200 Plate Handler and a plate adaptor
45-0472INT ECM 830 High Throughput Electroporation System with Monitoring ECM 830 Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of 30 (10 each), Cuvette Rack 660, ENHANCER 3000 Probe, ENHANCER Interface Box, Oscilloscope, Cables, 25-Well Plates (2 mm gap, 6X), HT-200 Plate Handler and a plate adaptor

 

SPECIFICATIONS FOR THE ECM 830 GENERATOR

Display Type 20-character by 4-line liquid crystal display; LED backlit
Power Source Voltage 100 to 240 VAC, 50 to 60 Hz, CAT II Power 500 W (Pulsed), 50 W (Idle)
Fusing 2.5 A, T rating 250 V
Environmental Characteristics 
Intended Use Indoor use only
Operating Temperature 10°C to 40°C
Cooling Convection through metal case
Relative Humidity 60%
Altitude 2,000 m (operating)

Mechanical Characteristics 
Footprint 12.5" x 12.25" x 5.5" (W x D x H)
Weight 15.0 lb (6.8 kg)
Controls Single rotary knob with push button toggle to set parameters, On/Off power and Start switches

 

STANDARD CAPABILITIES

Operational Status Internal self test upon start-up
Interface Digital User Interface
Input 110 V / 220 V Universal
Charge Time 5 sec maximum (without delay)
Pulse Length Range 10 μs – 999 μs LV Mode/ 1 μs resolution 1 msec – 999 msec LV Mode/ 1 msec resolution 1 sec – 10 sec LV Mode/ 0.1 sec resolution 10 μs – 600 μs HV Mode/ 1 μs resolution
Voltage Range 5 – 500 V LV Mode/ 1 V resolution 505 – 3,000 V HV Mode/ 5 V resolution
Multiple Pulsing 1 – 99
Pulse Interval 100 msec – 10 sec
Programmability Storage for 3 protocol setups (V, t, n,interval)
Arc Control Arc Quenching™
Safety Generator short circuit proof
Capacitance 4,000 μF LV, 111 μF HV
Input 500 A limit at 100 μs
Remote Operation Footswitch available

 

 

  • Mammalian cell transfection
  • In vitro, in vivo, ex vivo and in ovo tissue transfection
  • Nuclear transfer
  • Ex plant tissue transfection
  • Protoplast transfection
  • siRNA libraries
  • High throughput 25- and 96-well electroporation
  • CRISPR transfections
Item # Product
45-0167 2-Needle Array Kit, 10 mm, Pkg. of 6, with Handle
45-0168 2-Needle Array Kit, 5 mm, Pkg. of 6, with Handle
45-0465 25-Well Plate Adaptor for Plate Handlers
45-0450-M 96-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, 1 plate
45-0450 96-Well Disposable Electroporation Plate, 2 mm gap, 125 µl, Coated, 1 plate
45-0452 96-Well Disposable Electroporation Plates, 4 mm gap, 250 µl, 1 plate
45-0531 Adherent Cell Electrode Kit, 5 mm gap, includes 45-0204 cable
45-0530 Adherent Cell Electrode, 5 mm gap
45-0134 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/10, Gray
45-0124 Cuvette Plus, 1 mm gap, 90 µl, Sterile Pkg/50, Gray
45-0135 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/10, Blue
45-0125 Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue
45-0125INT Cuvette Plus, 2 mm gap, 400 µl, Sterile Pkg/50, Blue
45-0136 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/10, Yellow
45-0126 Cuvette Plus, 4 mm gap, 800 µl, Sterile Pkg/50, Yellow
45-0208 Cuvette Rack, 20 numbered positions
45-0211 ECM 830 Foot Pedal
45-0059 Enhancer 3000SC with Interface Box, Oscilloscope, Communications Module and Cables (N. America)
45-0108 Flat Electrode for Cell Fusion
45-0110 Flatpack Chambers, 0.56 mm gap, 80 µl volume, pkg. of 50
45-0109 Flatpack Chambers, 1.83 mm gap, 1.5 ml volume, pkg. of 50
45-0161 Genetrodes Kit, 10 mm Straight (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0162 Genetrodes Kit, 5 mm L-Shape (GOLD TIP) with 45-203 holder and 45-0216 cables
45-0160 Genetrodes Kit, 5 mm Straight (GOLD TIP), with 45-0203 holder and 45-0216 cables
45-0117 Genetrodes, 1 mm L-Shape (GOLD TIP)
45-0164 Genetrodes, 1 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0114 Genetrodes, 10 mm Straight (GOLD TIP)
45-0116 Genetrodes, 3 mm L-Shape (GOLD TIP)
45-0163 Genetrodes, 3 mm L-Shape (GOLD TIP) with 45-0203 holder and 45-0216 cables
45-0115 Genetrodes, 5 mm L-Shape (GOLD TIP)
45-0113 Genetrodes, 5 mm Straight (GOLD TIP)
45-0106 Glass Microslides with rectangular electrodes, 10 mm gap, 2.0 ml, Pkg./1
45-0105 Glass Microslides with rectangular electrodes, 3.2 mm gap, 650 µl, Pkg/1
45-0103 Glass Microslides with round wire electrodes, 0.5 mm gap, 20 µl, Pkg/10
45-0104 Glass Microslides with round wire electrodes, 1.0 mm gap, 40 µl, Pkg/10
45-0400INT HT 100 Plate Handler, Manual Track Switching
45-0400 HT 100 Plate Handler, Manual Track Switching (N. America)
45-0401INT HT-200 Plate Handler, Auto-Sense Track Switching
45-0401 HT-200 Plate Handler, Auto-Sense Track Switching (N. America)
45-0107 Meander Fusion Chamber
45-0510 Needle L-Shaped Platinum Electrode Kit, 3 mm, with cables
45-0509 Needle L-Shaped Platinum Electrode, 3 mm
45-0495 Oocyte Electrode, Platinum Plated, 10 mm, 1 mm gap (Electrode only)
45-0496 Oocyte Electrode Kit, Platinum Plated, 10 mm, 1 mm gap, (with cables)
45-0100 Petri Dish Electrode
45-0501 Petri Dish Platinum Electrode Chamber, 10 mm, negative
45-0506 Petri Dish Platinum Electrode Chamber, 15 mm gap
45-0504 Petri Dish Platinum Electrode Chamber, 5 mm gap
45-0491 Petri Dish Platinum Electrode Chamber, 7 mm, negative
45-0507 Petri Dish Platinum Electrode for Tissues Chamber Kit, 15 mm gap
45-0505 Petri Dish Platinum Electrode for Tissues Chamber Kit, 5 mm gap
45-0513 Petri Dish Tissue Chamber Kit, 15 mm gap with cables
45-0130 Petri Pulser
45-0525 Platinum Tweezertrode Kit, 1 mm Flat (includes 45-0204 cable)
45-0486 Platinum Tweezertrode, 1 mm Diameter (includes 45-0204 cables)
45-0524 Platinum Tweezertrode, 1 mm Flat
45-0487 Platinum Tweezertrode, 3 mm Diameter (includes 45-0204 cable)
45-0489 Platinum Tweezertrode, 5 mm Diameter (includes 45-0204 cables)
45-0488 Platinum Tweezertrode, 7 mm Diameter (includes 45-0204 cables)
45-0207 Safety Stand, Adjustable Gap (for use with Cuvettes)
45-0119 Stainless Tweezertrode Electrode, 10 mm Diameter
45-0118 Stainless Tweezertrode Electrode, 7 mm Diameter
45-0166 Stainless Tweezertrode Kit, 10 mm (includes 45-0204 cable)
45-0165 Stainless Tweezertrode Kit, 7 mm (includes 45-0204 cable)
45-0493 Triple Electrode Tweezertrode, 3 mm
45-0494 Triple Electrode Tweezertrode, 5 mm

ECM 830 User's Manual

 

ECM 830 HT Technical Specifications

 

CRISPR Brochure

 

REFERENCES

  

Xiaohong Liu, Corbett T Berry, Gordon Ruthel, Jonathan J. Madara, Katelyn MacGillivray, Carolyn M. Gray, Lisa A. Madge , Kelly A. McCorkell, Daniel P. Beiting, Uri Hershberg, Michael J. May and Bruce D. Freedman., T Cell Receptor-Induced NF-B Signaling and Transcriptional Activation Are Regulated by STIM1- and Orai1-Mediated Calcium Entry. The American Society for Biochemistry and Molecular Biology. 2016.

 

Mehul S. Patel, Jen Lee, Manuel Baz, Claire E. Wells, Susannah Bloch, Amy Lewis, Anna V. Donaldson, Benjamin E. Garfield , Nicholas S. Hopkinson, Amanda Natanek, William D-C Man, Dominic J. Wells, Emma H. Baker, Michael I. Polkey & Paul R. Kemp., Growth differentiation factor-15 is associated with muscle mass in chronic obstructive pulmonary disease and promotes muscle wasting in vivo. Journal of Cachexia, Sarcopenia and Muscle. 2016.

 

Jan Baumgart, Nadine Grebe., C57BL/6-specific conditions for efficient in utero electroporation of the central nervous system. J Neurosci Methods. 2015 Jan 30.

 

Shi, W. et al., Generation of sp3111 transgenic RNAi mice via permanent integration of small hairpin RNAs in repopulating spermatogonial cells in vivo, 2010. Acta Biochim Biophys Sin, Volume 42, Issue 2.

 

Nasser, N. et al., Alternatively spliced Spalax heparanase inhibits extracellular matrix degradation, tumor growth, and metastasis, 2009, PNAS.

 

Clape, C. et al., miR-143 Interferes with ERK5 Signaling, and Abrogates Cancer Progression in Mice, 2009, PLOS ONE.

 

Nyugen, K. et al., T Cell Costimulation via the Integrin VLA-4 Inhibits the Actin-Dependent Centralization of Signaling Microclusters Containing the Adaptor SLP-76, 2008, Immunity.

 

Lin, G. et. al, Regeneration of neural crest derivatives in the Xenopus tadpole tail, 2007, BMC Developmental Biology.

 

Chalberg, T. et al., phiC31 Integrase Confers Genomic Integration and Long-Term Transgene Expression in Rat Retina, June 2005, Investigative Ophthalmology & Visual Science, Vol. 46, No. 6.

 

Deora, A. et al., Efficient Electroporation of DNA and Protein into Confluent and Differentiated Epithelial Cells in Culture, 2007, Traffic