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- ECM 830 High Throughput Electroporation System
ECM 830 High Throughput Electroporation System
The BTX® High Throughput (HT) System, offers a multi-well electroporation technology for processing multiple samples in seconds. Using the HT Multi-Well plates instead of traditional cuvettes, the researcher can transition from a single cuvette to either a 96 well or 25 well electroporation plate by using one simple plate handler. This increases yields and the number of experiment runs in a single day. Experiments take seconds to run, allowing for quick and efficient optimization of the
electrical and biological parameters. Once optimized, samples are rapidly processed increasing yields and saving valuable time and money. The HT plate handler is compatible with the ECM 830 and some older ECM 830 generators (for more
details regarding compatibility of your ECM 830 systems please contact BTX Tech Support). The HT System is comprised of 3 components: The HT Multi-Well Plates, an HT Plate Handler and the BTX ECM 830 Generator.
APPLICATIONS
- Mammalian Cells
- Primary Cells
- Stem Cells
- siRNA Libraries
THE HT-100/HT-200
Standard Capabilities:
| Power |
100 to 240 VAC, 50/60 Hz, 15 W, 0.50 A fuse (2)
|
| Voltage Range | 0 to 3000V DC (Pulse) |
| Pulse Length Range | 10 μsec to 10 sec |
| Pulse Number Range | 1 to 99 |
| Operating Temperature | 5° to 40°C |
| Intended Use | Indoor Use |
| Relative Humidity | 20 to 80% |
| Maximum Altitude | 2,000 m (6,562 ft) |
| Pollution Degree | II |
| Insulation Degree | CAT I |
| Physical Characteristics for Models HT-100 and HT-200: | |
| Width x Depth x Height | 23 x 21.5 x 14 cm (9.1 x 8.5 x 5.5 in) |
| Weight | 4.8 Kg (10.6 lbs) |
THE ECM® 830 GENERATOR
Standard Capabilities:
| Display |
Type: 20-character by 4-line liquid crystal display. LED backlit.
|
| Power Source Voltage | 100 to 240 Vac, 50 to 60 Hz,CAT II |
| Power | Pulse: 500 W Idle: 150 W |
| Fusing | T2.5 250 V |
| Environmental Characteristics: | |
| Intended Use | 10°C to + 40°C |
| Cooling | Convection through metal case |
| Relative Humidity | 90% relative humidity |
| Altitude | 2,000 m (operating) |
| Mechanical Characteristics: | |
| Footprint | 12.5 x 12.25 x 5.5 in (31.75 x 3.12 x 13.97 cm) (W x D x H) |
| Weight | 15 lbs (6.8 kg) |
| Controls | Single rotary encoder with integrated push button |
| Electrical Characteristics: | |
| Voltage | 5 to 500 Volts (LV mode)@1 Volt resolution 30 to 3000 Volts (HV mode)@5 Volts resolution (Voltage Delivery@5% accuracy and Voltage Monitoring@5.0% accuracy) |
Order # Product
45-0421 Includes ECM 830 Generator, 4 mm gap, 2 x 96-Well
Plates, Plate Seals and HT-200 Plate Handler
45-0411 Includes ECM 830 Generator, 4 mm gap, 6 x 25-Well
Plates, Plate Seals and HT-200 Plate Handler
45-0452 96-Well Disposable Electroporation Plates, 4 mm gap,
250 μl, 1 plate
45-0450 96-Well Disposable Electroporation Plate, 2 mm gap,
125 μl, 1 plate
45-0462 25-Well Disposable Electroporation Plate, 4 mm gap,
250 μl, 1 plate
45-0466 25-Well Disposable Electroporation Plate, 2 mm gap,
125 μl, 1 plate
45-0463 25-Well Disposable Electroporation Plate, 4 mm,
250 μl, pkg. of 6
45-0467 25-Well Disposable Electroporation Plates, 2 mm,
125 μl, pkg. of 6
45-0401 HT-200 Plate Handler, Automatic Column Switching
BTX Monthly Tech Trends Articles
For further references regarding specific applications and optimization, please contact BTX Technical Support:
BTX-Division of Harvard Apparatus
84 October Hill Road
Holliston, MA 01746
Phone: 1-508-893-8999
Toll Free: 1-800-272-2775
Fax: 1-508-429-5732
Email: techsupport.btx@harvardapparatus.com
If outside the United States or Canada: call 508-893-8999 or
contact your nearest BTX Distributor
Blackmore, M.G., et al., High content screening of cortical neurons identifies novel regulators of axon growth, 2010, Molecular and Cellular Neuroscience
Buchser, W. J. et. al., 96-Well electroporation method for transfection of mammalian central neurons, 2006, BioTechniques
Zhao, Y. et. al., High-Efficiency Transfection of Primary Human and Mouse T Lymphocytes Using RNA Electroporation, 2006, Molecular Therapy
