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ECM 830 for In Vivo Applications

  • ECM 830 Square Wave Electroporator
  • Recommended accessory: Footswitch (45-0211)
  • Recommended accessory: Tweezertrode
SKU:
ECM_830_for_In_Vivo_Applications



FEATURES

  • A wide range of voltages from 5 to 3000 V
  • Finer voltage discrimination
  • Pulse durations from 10 μsec to 10 sec
  • Arc Quenching
  • Digital display of actual voltage and pulse
  • length delivered

APPLICATIONS

  • Mammalian Cells Transfection
  • In Vitro, In Vivo, Ex Vivo & In Ovo Tissue Transfection
  • Nuclear Transfer
  • Plant Protoplast Transfection
  • High Throughput 25/96 Well Electroporation

The ECM® 830 is a Square Wave Pulse generator designed for in vitro and in vivo electroporation applications. The versatility of the ECM® 830 applications for gene, drug and protein delivery include; mammalian cells, in vivo and ex vivo tissues, zebra fish tissue and embryos, nuclear transfer, embryo manipulation, plant protoplast and basic bacteria and yeast transformations. The ECM® 830 can be used in combination with a wide array of BTX® specialty electrodes and accessories to enhance your molecular and drug delivery experiments.

 

Standard Capabilities

 
Operational Status Internal self test upon start-up
Interface Digital User Interface
Input 110 V/220 V Universal
Charge Time 5 sec maximum (without delay) 
Pulse Length
Range 10 μs – 999 μs LV Mode/ 1 μs resolution
1 msec – 999 msec LV Mode/ 1 msec
resolution 1 sec – 10 sec LV Mode/
0.1 sec resolution 10 μs – 600 μs HV
Mode/ 1 μs resolution
Voltage Range
5 – 500 V LV Mode/ 1 V resolution 505 –
3000 V HV Mode/ 5 V resolution
Multiple Pulsing  1 – 99
Pulse Interval 100 msec – 10 sec 
Programmability  Storage for 3 protocol setups (V,t,n,interval)
Arc Control Arc Quenching™
Safety Generator short circuit proof
Capacitance 4000 μF LV, 111 μF HV
Input 500 A limit at 100 μs
Remote Operation Footswitch available

Order #        Product

45-0052         ECM® 830 Generator Only

45-0211       1250FS Footswitch for ECM 830

In Vivo Electrodes

Invasive Electrodes

45-0167

2-NEEDLE ARRAY KIT, 10mm (450120) (Includes handle (450205) ( and 2-needle array pk of 6)

45-0205

2-NEEDLE ARRAY HANDLE, 10mm          

45-0120

2-NEEDLE ARRAY , 10 mm, Pk OF 6                                            

45-0168

2-NEEDLE ARRAY KIT, 5mm (450121) (Includes handle (450206) and 2-needle array Pk of 6)

45-0206

2-NEEDLE ARRAY HANDLE, 5mm                                      

45-0121

2-NEEDLE ARRAY , 5mm, PK OF 6                                          

45-0510

NEEDLE L-SHAPED PLATINUM ELECTRODE KIT, 3mm INCLUDES: 2 Extended L-Shaped Platinum Needles Ultra thin Electrodes and connecting cables

450-509

NEEDLE L-SHAPED PLATINUM ELECTRODE 3mm, 2 Needle electrodes L-shaped ultra thin diameter platinum (needs 45-0508 cables)

45-0113

GENETRODES, STRAIGHT (GOLD TIP) 5mm (need 45-0203 holder and 45-0216 cables)

45-0160

GENETRODE (450113), STRAIGHT, GOLD TIP (includes holder (45-0203) and Connection cable banana plug to micro grabber (45-0216))

45-0114

GENETRODES, STRAIGHT (GOLD TIP) 10mm (need 45-0203 holder and 45-0216 cables)

45-0161

GENETRODE (450114) STRAIGHT, GOLD TIP, 10mm (includes holder (45-0203) and cables (45-0216))

 

 

Non Invasive Electrodes

45-0101

CALIPER ELECTRODE, 1.0CM X 1.0CM Transdermal delivery applications

45-0102

CALIPER ELECTRODE, 2.0 cm x 2.0 cm and extra 1.5CM X 1.5CM electrodes included Electrochemotherapy applications

45-0486

TWEEZERTRODES KIT, 1mm Platinum (includes cables 45-0204)

45-0487

TWEEZERTRODES KIT, 3mm Platinum (includes cables 45-0204)

45-0489

TWEEZERTRODES KIT, 5mm Platinum (includes cables 45-0204)

45-0488

TWEEZERTRODES KIT, 7mm Platinum (includes cables 45-0204)

45-0165

TWEEZERTRODE KIT, 7mm (45-0118)Stainless Steel

45-0118

TWEEZERTRODE ONLY, 7mm Stainless Steel                                        

45-0166

TWEEZERTRODE KIT, 10mm (450119)Stainless Steel

45-0119

TWEEZERTRODE ONLY, 10mm Stainless Steel                                      

45-0122

GENEPADDLES, 3X5mm

45-0169

GENEPADDLE KIT, 3X5mm (45-0123) (includes holder (45-0203) and cables (45-0216)

45-0123

GENEPADDLES, 5X7mm

45-0170

GENEPADDLE KIT, 5X7mm (45-0122) (includes holder (45-0203) and cables (45-0216)

45-0115

GENETRODES, L-SHAPE (GOLD TIP) 5mm (need 45-203 holder, and 45-0216 cable)

45-0162

GENETRODES L-SHAPE (450115), BENT, GOLD TIP, 5mm (includes holder (45-0203) and cables (45-0216))

45-0116

GENETRODES, L-SHAPE (GOLD TIP) 3mm (need 45-0203 holder and 45-0216 cable)

45-0163

GENETRODE, L-SHAPE (450116), BENT, GOLD TIP,3mm(includes holder (45-0203) and cables (45-0216))

45-0203

GENETRODES/GENEPADDLE HOLDER WITH SHAFT            

45-0117

GENETRODES, L-SHAPE (GOLD TIP)1mm(need 45-0203 holder and 45-0216 cables)

45-0164

GENETRODES, L-SHAPE (450117), BENT, GOLD TIP,1mm (includes holder (45-0203) and cables (45-0216))

45-0216

CONNECTION CABLE, Banana Plug To Micro grabber Plug, 10 FT

45-0204

TWEEZERTRODE CABLE (for use with all Tweezertrodes)

BTX Protocol Database

BTX Monthly Tech Trends Articles

The BTX Current

In Vivo Tutorials

User's Manual

 

For further references regarding specific applications and optimization, please contact BTX Technical Support:

 

BTX-Division of Harvard Apparatus

84 October Hill Road

Holliston, MA 01746

Phone: 1-508-893-8999

Toll Free: 1-800-272-2775

Fax: 1-508-429-5732

Email: techsupport.btx@harvardapparatus.com

 

If outside the United States or Canada: call 508-893-8999 or

contact your nearest BTX Distributor

Shi, W. et al., Generation of sp3111 transgenic RNAi mice via permanent integration of small hairpin RNAs in repopulating spermatogonial cells in vivo, 2010, Acta Biochim Biophys Sin, Volume 42, Issue 2


Nasser, N. et al., Alternatively spliced Spalax heparanase inhibits extracellular matrix degradation, tumor growth, and metastasis, 2009, PNAS

 

Clape, C. et al., miR-143 Interferes with ERK5 Signaling, and Abrogates Prostate Cancer Progression in Mice, 2009, PLOS ONE

 

Nyugen, K. et al., T Cell Costimulation via the Integrin VLA-4 Inhibits the Actin-Dependent Centralization of Signaling Microclusters Containing the Adaptor SLP-76, 2008, Immunity

 

Lin, G. et. al, Regeneration of neural crest derivatives in the Xenopus tadpole tail, 2007, BMC Developmental Biology

 

Chalberg, T. et al., C31 Integrase Confers Genomic Integration and Long-Term Transgene Expression in Rat Retina, June 2005, Investigative Ophthalmology & Visual Science, Vol. 46, No. 6

 

Deora, A. et al., Efficient Electroporation of DNA and Protein into Confluent and Differentiated Epithelial Cells in Culture, 2007, Traffic



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