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- ECM® 830 for In Vivo Applications
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- In Vivo Transfection
- ECM® 830 for In Vivo Applications
ECM® 830 for In Vivo Applications
FEATURES
- A wide range of voltages from 5 to 3000 V
- Finer voltage discrimination
- Pulse durations from 10 μsec to 10 sec
- Arc Quenching
- Digital display of actual voltage and pulse
- length delivered
APPLICATIONS
- Mammalian Cells Transfection
- In Vitro, In Vivo, Ex Vivo & In Ovo Tissue Transfection
- Nuclear Transfer
- Plant Protoplast Transfection
- High Throughput 25/96 Well Electroporation
The ECM® 830 is a Square Wave Pulse generator designed for in vitro and in vivo electroporation applications. The versatility of the ECM® 830 applications for gene, drug and protein delivery include; mammalian cells, in vivo and ex vivo tissues, zebra fish tissue and embryos, nuclear transfer, embryo manipulation, plant protoplast and basic bacteria and yeast transformations. The ECM® 830 can be used in combination with a wide array of BTX® specialty electrodes and accessories to enhance your molecular and drug delivery experiments.
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Standard Capabilities |
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| Operational Status | Internal self test upon start-up |
| Interface | Digital User Interface |
| Input | 110 V/220 V Universal |
| Charge Time | 5 sec maximum (without delay) |
| Pulse Length |
Range 10 μs – 999 μs LV Mode/ 1 μs resolution
1 msec – 999 msec LV Mode/ 1 msec
resolution 1 sec – 10 sec LV Mode/
0.1 sec resolution 10 μs – 600 μs HV
Mode/ 1 μs resolution
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| Voltage Range |
5 – 500 V LV Mode/ 1 V resolution 505 –
3000 V HV Mode/ 5 V resolution
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| Multiple Pulsing | 1 – 99 |
| Pulse Interval | 100 msec – 10 sec |
| Programmability | Storage for 3 protocol setups (V,t,n,interval) |
| Arc Control | Arc Quenching™ |
| Safety | Generator short circuit proof |
| Capacitance | 4000 μF LV, 111 μF HV |
| Input | 500 A limit at 100 μs |
| Remote Operation | Footswitch available |
Order # Product
45-0052 ECM® 830 Generator Only
45-0211 1250FS Footswitch for ECM 830
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In Vivo Electrodes |
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Invasive Electrodes |
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45-0167 |
2-NEEDLE ARRAY KIT, 10mm (450120) (Includes handle (450205) ( and 2-needle array pk of 6) |
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45-0205 |
2-NEEDLE ARRAY HANDLE, 10mm |
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45-0120 |
2-NEEDLE ARRAY , 10 mm, Pk OF 6 |
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45-0168 |
2-NEEDLE ARRAY KIT, 5mm (450121) (Includes handle (450206) and 2-needle array Pk of 6) |
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45-0206 |
2-NEEDLE ARRAY HANDLE, 5mm |
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45-0121 |
2-NEEDLE ARRAY , 5mm, PK OF 6 |
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45-0510 |
NEEDLE L-SHAPED PLATINUM ELECTRODE KIT, 3mm INCLUDES: 2 Extended L-Shaped Platinum Needles Ultra thin Electrodes and connecting cables |
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450-509 |
NEEDLE L-SHAPED PLATINUM ELECTRODE 3mm, 2 Needle electrodes L-shaped ultra thin diameter platinum (needs 45-0508 cables) |
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45-0113 |
GENETRODES, STRAIGHT (GOLD TIP) 5mm (need 45-0203 holder and 45-0216 cables) |
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45-0160 |
GENETRODE (450113), STRAIGHT, GOLD TIP (includes holder (45-0203) and Connection cable banana plug to micro grabber (45-0216)) |
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45-0114 |
GENETRODES, STRAIGHT (GOLD TIP) 10mm (need 45-0203 holder and 45-0216 cables) |
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45-0161 |
GENETRODE (450114) STRAIGHT, GOLD TIP, 10mm (includes holder (45-0203) and cables (45-0216)) |
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Non Invasive Electrodes |
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45-0101 |
CALIPER ELECTRODE, 1.0CM X 1.0CM Transdermal delivery applications |
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45-0102 |
CALIPER ELECTRODE, 2.0 cm x 2.0 cm and extra 1.5CM X 1.5CM electrodes included Electrochemotherapy applications |
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45-0486 |
TWEEZERTRODES KIT, 1mm Platinum (includes cables 45-0204) |
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45-0487 |
TWEEZERTRODES KIT, 3mm Platinum (includes cables 45-0204) |
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45-0489 |
TWEEZERTRODES KIT, 5mm Platinum (includes cables 45-0204) |
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45-0488 |
TWEEZERTRODES KIT, 7mm Platinum (includes cables 45-0204) |
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45-0165 |
TWEEZERTRODE KIT, 7mm (45-0118)Stainless Steel |
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45-0118 |
TWEEZERTRODE ONLY, 7mm Stainless Steel |
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45-0166 |
TWEEZERTRODE KIT, 10mm (450119)Stainless Steel |
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45-0119 |
TWEEZERTRODE ONLY, 10mm Stainless Steel |
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45-0122 |
GENEPADDLES, 3X5mm |
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45-0169 |
GENEPADDLE KIT, 3X5mm (45-0123) (includes holder (45-0203) and cables (45-0216) |
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45-0123 |
GENEPADDLES, 5X7mm |
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45-0170 |
GENEPADDLE KIT, 5X7mm (45-0122) (includes holder (45-0203) and cables (45-0216) |
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45-0115 |
GENETRODES, L-SHAPE (GOLD TIP) 5mm (need 45-203 holder, and 45-0216 cable) |
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45-0162 |
GENETRODES L-SHAPE (450115), BENT, GOLD TIP, 5mm (includes holder (45-0203) and cables (45-0216)) |
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45-0116 |
GENETRODES, L-SHAPE (GOLD TIP) 3mm (need 45-0203 holder and 45-0216 cable) |
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45-0163 |
GENETRODE, L-SHAPE (450116), BENT, GOLD TIP,3mm(includes holder (45-0203) and cables (45-0216)) |
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45-0203 |
GENETRODES/GENEPADDLE HOLDER WITH SHAFT |
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45-0117 |
GENETRODES, L-SHAPE (GOLD TIP)1mm(need 45-0203 holder and 45-0216 cables) |
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45-0164 |
GENETRODES, L-SHAPE (450117), BENT, GOLD TIP,1mm (includes holder (45-0203) and cables (45-0216)) |
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45-0216 |
CONNECTION CABLE, Banana Plug To Micro grabber Plug, 10 FT |
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45-0204 |
TWEEZERTRODE CABLE (for use with all Tweezertrodes) |
BTX Monthly Tech Trends Articles
For further references regarding specific applications and optimization, please contact BTX Technical Support:
BTX-Division of Harvard Apparatus
84 October Hill Road
Holliston, MA 01746
Phone: 1-508-893-8999
Toll Free: 1-800-272-2775
Fax: 1-508-429-5732
Email: techsupport.btx@harvardapparatus.com
If outside the United States or Canada: call 508-893-8999 or
contact your nearest BTX Distributor
Shi, W. et al., Generation of sp3111 transgenic RNAi mice via permanent integration of small hairpin RNAs in repopulating spermatogonial cells in vivo, 2010, Acta Biochim Biophys Sin, Volume 42, Issue 2
Nasser, N. et al., Alternatively spliced Spalax heparanase inhibits extracellular matrix degradation, tumor growth, and metastasis, 2009, PNAS
Clape, C. et al., miR-143 Interferes with ERK5 Signaling, and Abrogates Prostate Cancer Progression in Mice, 2009, PLOS ONE
Nyugen, K. et al., T Cell Costimulation via the Integrin VLA-4 Inhibits the Actin-Dependent Centralization of Signaling Microclusters Containing the Adaptor SLP-76, 2008, Immunity
Lin, G. et. al, Regeneration of neural crest derivatives in the Xenopus tadpole tail, 2007, BMC Developmental Biology
Chalberg, T. et al., C31 Integrase Confers Genomic Integration and Long-Term Transgene Expression in Rat Retina, June 2005, Investigative Ophthalmology & Visual Science, Vol. 46, No. 6
Deora, A. et al., Efficient Electroporation of DNA and Protein into Confluent and Differentiated Epithelial Cells in Culture, 2007, Traffic
