Ex vivo electroporation transfection in tissue with ECM 830

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Ex Plant Tissue
ECM® 830 for Ex Plant Tissue Transfections

ECM® 830 for Ex Plant Tissue Transfections

FEATURES

A wide range of voltages from 5 to 3000 V
Finer voltage discrimination
Pulse durations from 10 μsec to 10 sec
Arc Quenching
Digital display of actual voltage and pulse
length delivered

APPLICATIONS

Mammalian Cells Transfection
In Vitro, In Vivo, Ex Vivo & In Ovo Tissue Transfection
Nuclear Transfer
Plant Protoplast Transfection
High Throughput 25/96 Well Electroporation

The ECM® 830 is a Square Wave Pulse generator designed for in vitro and in vivo electroporation applications. The versatility of the ECM^® 830 applications for gene, drug and protein delivery include; mammalian cells, in vivo and ex vivo tissues, zebra fish tissue and embryos, nuclear transfer, embryo manipulation, plant protoplast and basic bacteria and yeast transformations. The ECM® 830 can be used in combination with a wide array of BTX® specialty electrodes and accessories to enhance your molecular and drug delivery experiments.

Specifications
Ordering Info
Technical
References

Standard Capabilities
Operational Status	Internal self test upon start-up
Interface	Digital User Interface
Input	110 V/220 V Universal
Charge Time	5 sec maximum (without delay)
Pulse Length	Range 10 μs – 999 μs LV Mode/ 1 μs resolution 1 msec – 999 msec LV Mode/ 1 msec resolution 1 sec – 10 sec LV Mode/ 0.1 sec resolution 10 μs – 600 μs HV Mode/ 1 μs resolution
Voltage Range	5 – 500 V LV Mode/ 1 V resolution 505 – 3000 V HV Mode/ 5 V resolution
Multiple Pulsing	1 – 99
Pulse Interval	100 msec – 10 sec
Programmability	Storage for 3 protocol setups (V,t,n,interval)
Arc Control	Arc Quenching™
Safety	Generator short circuit proof
Capacitance	4000 μF LV, 111 μF HV
Input	500 A limit at 100 μs
Remote Operation	Footswitch available

Order #	Product
45-0052	ECM® 830 Generator Only
45-0211	1250FS Footswitch for ECM 830

Ex Plant Electrodes
45-0490	TISSUE SLICE CHAMBER KIT INCLUDES; Tissue slice chamber 7mmx7mm, positive tissue electrode handle, connector cables for both chamber and electrode.
45-0491	TISSUE SLICE CHAMBER Glass Petri Dish with Platinum Chamber for tissue slice electroporation, 7mm x 7mm diameter (needs 45-0492,45-0503 and 450511 for operation)
45-0492	TISSUE SLICE POSITIVE ELECTRODE, Electrode handle for the positive connection of the tissue slice chamber (needs 45-0491, 45-0503 and 450511 for operation)
45-0500	TISSUE SLICE CHAMBER KIT INCLUDES; Tissue slice chamber 10mmx10mm, positive tissue electrode handle, connector cables for both chamber and electrode.
45-0501	TISSUE SLICE CHAMBER Glass Petri Dish with Platinum Chamber for tissue slice electroporation, 10mm x 10mm diameter (needs 45-0502,45-0503 and 45-0511 for operation)
45-0502	TISSUE SLICE POSITIVE ELECTRODE, Electrode handle for the positive connection of the tissue slice chamber (needs 45-0501, 45-0503 and 45-0504 for operation)
45-0505	PETRI DISH CHAMBER FOR TISSUE 5mm KIT INCLUDES: Glass Petri Dish with deep chamber 5mm x 5mm width and depth and micro grabber connection cables.
45-0504	PETRI DISH CHAMBER 5mm, glass petri dish with 5mm x 5mm deep chamber for tissue (needs 45-0216)
45-0506	PETRI DISH TISSUE CHAMBER ELECTRODE 15mm, tissue chamber electrode 15mm x 15mm deep. (needs 45-0216)
45-0507	PETRI DISH TISSUE CHAMBER 15mm KIT INCLUDES: Glass Petri Dish with deep chamber 15mm x15mm width and depth and micro grabber connection cables