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ECM® 830 for Adherent Cell Transfection

  • ECM 830 Square Wave Electroporator (45-0052)
  • Adherent cell electrode (45-0530)
  • Petri Pulser electrode (45-0130)
  • Footswitch (45-0211)



FEATURES

  • A wide range of voltages from 5 to 3000 V
  • Finer voltage discrimination
  • Pulse durations from 10 μsec to 10 sec
  • Arc Quenching
  • Digital display of actual voltage and pulse length delivered

The ECM® 830 coupled with the Petri Pulser Electrode or the Petri Dish electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in. The Petri Pulser is ideal for 6 well plates and the Petri Dish Electrode is ideal for 100mm petri dishes.

 

Standard Capabilities

 
Operational Status Internal self test upon start-up
Interface Digital User Interface
Input 110 V/220 V Universal
Charge Time 5 sec maximum (without delay) 
Pulse Length
Range 10 μs – 999 μs LV Mode/ 1 μs resolution
1 msec – 999 msec LV Mode/ 1 msec
resolution 1 sec – 10 sec LV Mode/
0.1 sec resolution 10 μs – 600 μs HV
Mode/ 1 μs resolution
Voltage Range
5 – 500 V LV Mode/ 1 V resolution 505 –
3000 V HV Mode/ 5 V resolution
Multiple Pulsing  1 – 99
Pulse Interval 100 msec – 10 sec 
Programmability  Storage for 3 protocol setups (V,t,n,interval)
Arc Control Arc Quenching™
Safety Generator short circuit proof
Capacitance 4000 μF LV, 111 μF HV
Input 500 A limit at 100 μs
Remote Operation Footswitch available

 

Order #          Product

45-0052         ECM® 830 Generator Only

 

Adherent Cell Electrodes

45-0130

PETRI PULSER ELECTRODE, for 6 well plates/35 mm wells

45-0100

PETRI DISH ELECTRODE 2mm gap 90 mm well dish

BTX Protocol Database

BTX Monthly Tech Trends Articles

The BTX Current

In Vivo Tutorials

 

For further references regarding specific applications and optimization, please contact BTX Technical Support:

 

BTX-Division of Harvard Apparatus

84 October Hill Road 

Holliston, MA 01746 

Phone: 1-508-893-8999

Toll Free: 1-800-272-2775

Fax: 1-508-429-5732

Email: techsupport.btx@harvardapparatus.com

 

If outside the United States or Canada: call 508-893-8999 or

contact your nearest BTX Distributor

 



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