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ECM® 630 Exponential Decay Wave Electroporation System

  • Image 1
  • Safety Stand, Cuvettes & Cuvette Rack
  • ECM 630 Electroporator



FEATURES

  • Utilizes the BTX Power Platform Technology design and novel digital user interface
  • Precision Pulse™ Technology provides unparalleled power in controlling the time constant
  • Delivers a maximum of 6000 A in the Low Voltage Mode; the ECM® 630 is the most powerful generator in its class
  • Voltage range of 10 V to 500 V with 1 V resolution and 1 μF, 25 μF to 3275 μF in 25 μF increments. 25 μF to 1575 μF, 25 μF resolution with “none” setting
  • Voltage range of 50 V to 2500 V with 5 V resolution and either 25 μF or 50 μF, 25 μF to 1575 μF with 25 μF resolution
  • Hundreds of BTX protocols may be duplicated with this instrument
  • The additional ECM® 630 resistor selection “none” will allow researchers to reproduce protocols from competitive systems
  • Can perform the widest range of electroporation applications among commercially available electroporation generators

The ECM® 630 is an Exponential Decay Wave electroporation generator providing a broad range of voltage and time constants for full flexibility in varying applications. The ability to select the resistance and capacitance values, and adjust the range of voltages, is the key to achieving the optimal time constants and field strengths needed for efficient transformation of prokaryote and eukaryote transfection. Flexibility is important to researchers, so BTX has designed a plug and play system for our ECM® 630 system to transition between standard cuvettes and to 25/96-well plates using the high throughput Plate Handler.

APPLICATIONS

  • Transformation of Bacteria and Yeast
  • Transfection of Mammalian Cells
  • Transfection of Plant Tissue & Plant Protoplasts
  • High Throughput 25/96-Well

96-WELL ELECTROPORATION

Transition from standard cuvettes to multi-well electroporation is quick and simple with the addition of the High Throughput (HT) plate handler and plates. The HT plate handler accommodates either 25/96 well electroporation plates and it operates with the ECM® 630 generator.

MONITORING OPTION

The ENHANCER 3000 allows the researcher to monitor and track key electrical parameters used in electroporation applications. The electrical pulse data is captured as both a graphic display of the wave form and electrical output values following each experiment.

COMBINATION SYSTEM

The combo system includes the power and flexibility of the ECM® 630 Exponential Decay Wave Generator  and the gentle strength and versatility of the ECM® 830 Square Wave System to provide the highest transformation and transfection efficiencies and viabilities in bacteria, mammalian cells and in vivo tissues. BTX offers the ECM® 630/ECM® 830 Combo System complete with two safety stands and sample cuvettes.


Operational Status Internal self test upon start-up
Interface Digital User Interface
Input 110 V/220 V Universal
Charge Time 5 sec maximum
Arc Control Arc Quenching™
Voltage Range 10 – 500 V LV Mode/ 1 V resolution 50 – 2500 V HV Mode/ 5 V resolution
Capacitance Range 1 μF, 25 μF to 3,275 μF LV Mode 25 μF, 50 μF HV Mode
Resistance Range 25 ohm – 1,575 ohm/ 25 ohm resolution HV & LV Modes “None” setting to simulate alternative systems lacking resistance control LV Mode
Catalog  # Product
45-0001
Electroporation System includes ECM 630 Generator,
630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm pkg. of
30 (10 each) and Cuvette Rack 660 pkg. of 30 (10 each)
and Cuvette Rack 660
45-0051 Generator Only
45-0412
Includes ECM 630 Generator, 6 x 25-Well Plates (2 mm),
Plate Seals, and HT-100 Plate Handler plates (4 mm) and
a plate adaptor
45-0422
Includes ECM 630 Generator, 2 x 96-Well Plates (2 mm),
Plate Seals and HT-100 Plate Handlerplates (4 mm) and a
plate adaptor
45-0061
Combination package includes ECM 830 Generator, ECM
630 Generator, 2 x Safety Stands, 30 Cuvettes (10 each:
1 mm, 2 mm and 4 mm) and Cuvette Rack
45-0071
ENHANCER 3000 Probe, ENHANCER Interface Box,
Oscilloscope, Communications Module, ECM 630
Generator, Safety Stand, 30 Cuvettes (10 each 1 mm,
2 mm and 4 mm) and Cables

BTX Protocol Database

BTX Monthly Tech Trends Articles

The BTX Current

In Vivo Tutorials

Technical Specifications

User's Manual

 

For further references regarding specific applications and optimization, please contact BTX Technical Support:

 

BTX-Division of Harvard Apparatus

84 October Hill Road 

Holliston, MA 01746 

Phone: 1-508-893-8999

Toll Free: 1-800-272-2775

Fax: 1-508-429-5732

Email: techsupport.btx@harvardapparatus.com

 

If outside the United States or Canada: call 508-893-8999 or

contact your nearest BTX Distributor

Kurth, M. et al. Reporter gene expression in cell culture stages and oocysts

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Lama, A. et al., Glyceraldehyde-3-Phosphate Dehydrogenase Is a Surface-

Associated, Fibronectin-Binding Protein of Trichomonas vaginalis, 2009,

Infection & Immunity


Thyagarajan, B. et al., Creation of Engineered Human Embryonic Stem Cell

Lines Using phiC31 Integrase, 2008, Stem Cell


Donoho, G. et al., Analysis of Gene Targeting and Intrachromosomal

Homologous Recombination Stimulated by Genomic Double-Strand Breaks

in Mouse Embryonic Stem Cells, 1998, Molecular & Cellular Biology



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