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ECM 399 Exponential Decay Wave Electroporation System

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  • PEP, Personal Electroporation Pak (45-0212)

  • Bacteria
  • Yeast
  • Fungus

The ECM® 399 is an exponential decay wave electroporation system specifically designed to deliver the field strengths and pulse lengths required for the simple transformation of bacteria and yeast cells. In low voltage mode the ECM® 399 has a limited capability for transfecting some mammalian cell lines. The ECM® 399 is ideal for basic transformation in research and academic environments. It is easy to operate, cost effective, compact in size and portable.


BTX provides a combination system which has the power of both its exponential decay and square wave technologies for labs involved in multiple applications. These labs need the versatility of the BTX systems to range from prokaryotes to eukaryotes with the ability to transfect delicate in vivo tissues efficiently. This combo system includes the power of the ECM® 399 Exponential Decay Wave generator to provide the highest transformation efficiencies of basic bacteria and yeast strains. This package option also includes the gentle strength and versatility of the ECM® 830 Square Wave System to provide high transfection efficiencies with equally high cell viabilities in mammalian cells and in vivo tissues. BTX offers the ECM® 399/ECM 830 Combo System complete with a PEP and a Safety Stand for cuvettes. These systems can be used together or separate as independent systems for operation in different labs with no extra components needed.

Display Type: 16-character liquid crystal LCD backlit
Power Source Voltage 100 to 240 Vac, 50 to 60 Hz, CAT I
Power < 100 W peak
Fusing 2.5 A, T rating 250 V
Operational Status Internal self test upon start-up
Interface Digital User Interface
Pulse Interval 100 msec – 10 sec
Charge Time 5 sec maximum

Voltage Range:

LV Mode 1,050 μF Fixed
HV Mode 36 μF Fixed
LV Mode 150 ohm Fixed
HV Mode 150 ohm
Maximum Voltage Output: 2,500 Volts Peak
Maximum Pulse Length 125 ms@500 Volts peak or 5 ms@2,500 Volts peak
Pollution Degree 2 (Not to be operated in conductive pollutants atmosphere)
Relative Humidity 60%
Altitude 2,000 m (operating)
Catalog # Product
45-0000 Electroporation System includes ECM® 399 Generator, PEP, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack 660
45-0050 ECM® 399 Generator Only
45-0060 Combination package includes ECM® 830 Generator, ECM® 399 Generator, Safety Stand, PEP, 30 Cuvettes, (10 each: 1 mm, 2 mm and 4 mm) and Cuvette Rack
45-0212 PEP Personal Electroporation Pak Cuvette Module

BTX Protocol Database

BTX Monthly Tech Trends Articles

The BTX Current 

Technical Specifications

User Manual


For further references regarding specific applications and optimization, please contact BTX Technical Support:


BTX-Division of Harvard Apparatus

84 October Hill Road

Holliston, MA 01746

Phone: 1-508-893-8999

Toll Free: 1-800-272-2775

Fax: 1-508-429-5732

Email: techsupport.btx@harvardapparatus.com


If outside the United States or Canada: call 508-893-8999 or

contact your nearest BTX Distributor

Hila Yehuda, et al., A transformation system for the biocontrol yeast, Candida oleophila, based on hygromycin B resistance, Current Genetics Vol. 40 pp. 282-287, 2001

Taghavi et al., Electroporation of Alcaligenes eutrophus with (Mega) Plasmids and Genomic DNA Fragments, Applied and Environmental Microbiology, 60(10), pgs 3585-3591, 1994

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