Loading... Please wait...Applications
New Products
Our Newsletter
- Home
- Suspension Cells
- ECM 399 Exponential Decay Wave Electroporation System
ECM 399 Exponential Decay Wave Electroporation System
- Bacteria
- Yeast
- Fungus
The ECM® 399 is an exponential decay wave electroporation system specifically designed to deliver the field strengths and pulse lengths required for the simple transformation of bacteria and yeast cells. In low voltage mode the ECM® 399 has a limited capability for transfecting some mammalian cell lines. The ECM® 399 is ideal for basic transformation in research and academic environments. It is easy to operate, cost effective, compact in size and portable.
BTX provides a combination system which has the power of both its exponential decay and square wave technologies for labs involved in multiple applications. These labs need the versatility of the BTX systems to range from prokaryotes to eukaryotes with the ability to transfect delicate in vivo tissues efficiently. This combo system includes the power of the ECM® 399 Exponential Decay Wave generator to provide the highest transformation efficiencies of basic bacteria and yeast strains. This package option also includes the gentle strength and versatility of the ECM® 830 Square Wave System to provide high transfection efficiencies with equally high cell viabilities in mammalian cells and in vivo tissues. BTX offers the ECM® 399/ECM 830 Combo System complete with a PEP and a Safety Stand for cuvettes. These systems can be used together or separate as independent systems for operation in different labs with no extra components needed.
| Display | Type: 16-character liquid crystal LCD backlit |
| Power Source Voltage | 100 to 240 Vac, 50 to 60 Hz, CAT I |
| Power | < 100 W peak |
| Fusing | 2.5 A, T rating 250 V |
| Operational Status | Internal self test upon start-up |
| Interface | Digital User Interface |
| Pulse Interval | 100 msec – 10 sec |
| Charge Time | 5 sec maximum |
|
Voltage Range: |
|
| LV Mode | 1,050 μF Fixed |
| HV Mode | 36 μF Fixed |
| Resistance: | |
| LV Mode | 150 ohm Fixed |
| HV Mode | 150 ohm |
| Maximum Voltage | Output: 2,500 Volts Peak |
| Maximum Pulse Length | 125 ms@500 Volts peak or 5 ms@2,500 Volts peak |
| Pollution Degree 2 | (Not to be operated in conductive pollutants atmosphere) |
| Relative Humidity | 60% |
| Altitude | 2,000 m (operating) |
| Catalog # | Product |
| 45-0000 | Electroporation System includes ECM® 399 Generator, PEP, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack 660 |
| 45-0050 | ECM® 399 Generator Only |
| 45-0060 | Combination package includes ECM® 830 Generator, ECM® 399 Generator, Safety Stand, PEP, 30 Cuvettes, (10 each: 1 mm, 2 mm and 4 mm) and Cuvette Rack |
| 45-0212 | PEP Personal Electroporation Pak Cuvette Module |
BTX Monthly Tech Trends Articles
For further references regarding specific applications and optimization, please contact BTX Technical Support:
BTX-Division of Harvard Apparatus
84 October Hill Road
Holliston, MA 01746
Phone: 1-508-893-8999
Toll Free: 1-800-272-2775
Fax: 1-508-429-5732
Email: techsupport.btx@harvardapparatus.com
If outside the United States or Canada: call 508-893-8999 or
contact your nearest BTX Distributor
Hila Yehuda, et al., A transformation system for the biocontrol yeast, Candida oleophila, based on hygromycin B resistance, Current Genetics Vol. 40 pp. 282-287, 2001
Taghavi et al., Electroporation of Alcaligenes eutrophus with (Mega) Plasmids and Genomic DNA Fragments, Applied and Environmental Microbiology, 60(10), pgs 3585-3591, 1994
