ECM 2001 System for Volumes up to 2mls

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Hybridoma Production
ECM 2001 System for Volumes up to 2mls

ECM 2001 System for Volumes up to 2mls

The ECM 2001 System is a multipurpose pulse generator system capable of performing both electrofusion for hybridoma production, hybrid cell formation and nuclear transfer applications in addition to electroporation of cells in suspension and in vivo applications. For use in hybridoma production applications, users would require the ECM 2001 generator, 3.2mm gap or 10mm gap Microslides, and connection cables. This system does not require proprietary fusion medium, but can be used with the Cytofusion Medium C for convenience.

Electrofusion is achieved by the generation of a proprietary 1 MHz AC waveform for benign dielectrophoretic cell alignment, microsecond switchover from AC to DC creates efficient fusion results. After fusion, the AC pulse reapplication maintains compression of the cells following the DC pulse resulting in a higher number of hybrids.

Specifications
Ordering Info
Technical
References

AC Parameters (Alignment)
Proprietary nonsinusoidal wave shape
Frequency	1 MHz
Voltage	0 – 75 V RMS (zero to peak)
Duration	0 – 99 sec
Post fusion AC	1/10 of pre fusion amplitude
Post fusion ramp	1 – 9 sec
Pause between AC/DC	50 μsec

DC Pulse Parameters (Fusion) (Electroporation)
High Voltage Mode
Voltage	10 – 3000 V
Pulse Length	1 – 99 μsec/ 1 μsec resolution
Low Voltage Mode:
Voltage	10 – 500 V
Pulse Length	1 – 9 pulses
Number of Pulses	0.01 – 0.99 msec/ 0.01 msec res 1 – 99 msec/ 1 msec res

Catalog Product

45-0018 ECM2001HP Hybridoma Production System includes the

                   ECM 2001 Generator, Micrograbber cables, Adapter set,

                   3.2 mm gap Microslide, 10 mm gap Microslide, and

                   Cytofusion Media (500 ml)

45-0080 ECM 2001 Generator Only

45-0216 Micrograbber Cables

45-0217 Banana to Banana Plug, 10 ft.

45-0089 Adapter Set Banana to Square Post

45-0088 Adapter Set F/F Banana to Square Splice

45-0087 Micrograbber Adapter

45-0107	MEANDER FUSION CHAMBER, .2mm GAP PK OF 4 (needs 45-0216 cables)
45-0103	MICROSLIDE 450 .5mm GAP, 20UL PK OF 10 (needs 45-0216 cable)
45-0104	MICROSLIDE 450-1 1mm GAP, 40UL PK OF 10 (needs 45-0216 cable)
45-0105	MICROSLIDE 453, 3.2 mm GAP 650ul (needs 45-0216 cable)
45-0106	MICROSLIDE 453-10, 10 mm GAP, 2.0ML (needs 45-0216 cable)