BTX offers advanced electroporation solutions for fast, efficient transfection of 2 to 20 mL of cell suspension, single cuvettes, or liter volumes. Specifically engineered for large-volume applications, the AgilePulse MAX and the AgilePulse HT system maximize cellular uptake with minimal heating and short cycle-time to ensure high cell viability in further cell processing.

Simple-to-use, cells and polynucleotide are suspended in our proprietary BTXpress Cytoporation^® medium and transferred via sterile syringe to either the large-volume electroporation chamber, cuvette, or sterile bags (depending on system), where a programmed sequence of electric pulses is applied. First, a sequence of short, high-intensity pulses opens pores in the cell membranes, followed by long, low-intensity pulses that drive the material into cells via electrophoresis. Our patented Pulse Agile^® technology optimizes these pulse parameters to maximize efficiency and cell viability.

Applications:

• Transfect cells such as bone marrow cells to produce or replace a missing protein
• Deliver siRNA to suppress gene expression
• Deliver genes for permanent gene correction
• Load cells with a drug for drug delivery
• Cancer immunotherapy
• Transfect eukaryotic cells for protein production in bioreactors
• Large-scale production of replication-deficient viruses