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Caliper Electrode

  • 1.0 x 1.0cm and 1.5 x 1.5cm Calipers
  • 1.5 x 1.5cm Caliper Electrode
  • 1.0 x 1.0cm Caliper Electrode



The BTX Caliper Electrodes are in vivo style re-usable electrodes used for a variety of applications. Caliper Electrodes are available in two sizes.  Each size consists of a caliper and a pair of electrode plates. The 1.0 x 1.0 cm electrode is used in smaller animals with a smaller tissue surface area, while the 2.0 x 2.0 cm is used to target slightly larger surface areas. The end plates of the Caliper Electrode may be adjusted by using the black roller mounted on the caliper. Caliper Electrodes may be cleaned with a mild detergent and sterilized with ethanol or ethylene oxide. Properly maintained Caliper Electrodes have an unlimited lifetime, and are compatible with the ECM 830 and ECM 2001 Electroporation Systems.

APPLICATIONS

  • Transdermal Tissue
  • Ex Vivo Tissues
  • In Vivo Muscle Tissue


Caliper Electrodes may be used for many electroporation applications, including drug and gene delivery. The electrodes span the target area and deliver electroporation pulses following injection of the molecule of interest. The electric field introduced by the Caliper Electrodes cause transient pores to form in the cells of the tissue, allowing uptake of the molecules into the cells. Prud'Homme et al. used the Caliper electrodes to deliver plasmid DNA to skin for gene therapy studies on diabetes.1 Mir et al. used the Caliper Electrode for in vivo skeletal muscle work2 as well as subcutaneous tumor electrochemotherapy applications3. Caliper Electrodes can potentially be used for intact plant work as well.4


 

Generator Compatibility ECM 830 and ECM 2001
Voltage Range
0 – 500 V (depending on electrode gap)
Pulse Length Range 10 μsec – 99 msec
Electrode Gap 0.1 to 13 cm
Electrode Dimensions 1 x 1 cm brass or 1.5 x 1.5 cm and 2 x 2 cm stainless steel


BTX Protocol Database

BTX Monthly Tech Trends Articles

The BTX Current

In Vivo Tutorials

User's Manual

 

For further references regarding specific applications and optimization, please contact BTX Technical Support:

 

BTX-Division of Harvard Apparatus

84 October Hill Road 

Holliston, MA 01746 

Phone: 1-508-893-8999

Toll Free: 1-800-272-2775

Fax: 1-508-429-5732

Email: techsupport.btx@harvardapparatus.com

 

If outside the United States or Canada: call 508-893-8999 or

contact your nearest BTX Distributor


Item # Plate Dimensions Material
45-0101 1 x 1 cm Brass
45-0102 1.5 x 1.5 cm and 2 x 2 cm Stainless Steel




1. Prud'Homme et al., Human Gene Therapy, 13: 395-406 (2002)

2. Mir et al., PNAS, 96, 4262-4267 (1999)

3. Mir et al., British Journal of Cancer, 77(12), 2336-2342 (1998)

4. Dev et al., U.S. Patent #5,859,327 Jan 12, 1999



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