• Efficient electroporation transfection
• High efficiency transfection of difficult cell ines
• High cell viability
• Low toxicity resulting in improved cell viability
• Single buffer for all cell types
• One buffer used in place of standard electroporation buffers for all mammalian cell types

BTXpress™ is a single buffer solution, developed to quickly and efficiently deliver genes into mammalian cells that were previously considered “hard to transfect” by chemical and other non-viral methods. This solution, in combination with BTX electroporators, provides researchers with the versatility needed for success across a broad range of cell types while maintaining critical cell viability. Transfection using this high performance electroporation solution is equally effective in delivering DNA as well as siRNA into mammalian cells.

The BTXpress™ solution offers increased numbers of transfections per kit compared to our competitors, providing better value to the researcher. As a universal solution, the BTXpress™ electroporation reagent can be used with competitor systems, including the Amaxa™ Nucleofector,^® to deliver similar to equal performance without the cost associated with other systems. The BTXpress™ High Performance Electroporation Solution is offered as kits which includes the BTX Plus cuvettes with transfer pipettes or as a buffer alone.

APPLICATIONS

Table 1: BTXpress™ “High Performance Electroporation Solution" vs. Amaxa™ Nucleofector®. Cells were electroporated with an EGFP reporter vector in parallel, using the BTX ECM 830 Square Wave Electroporator with the BTXpress™ High Performance Electroporation Solution or using the Amaxa™ (Lonza) system. EGFP expressing cells were identified 24hrs post-electroporation by flow cytometry and presented as a percentage of the live cell population.

Table 2: BTXpress™ Solution Transfection Efficiency vs. Amaxa™: Cells were electroporated in parallel with an EGFP reporter vector using either the BTX electroporation system with BTXpress™ High Performance Electroporation Solution or PBS. In comparison to the same cells transfected in the Amaxa™ (Lonza) system using the Amaxa™ kit V solution. The EGFP expressing cells were identified 24hrs post-electroporation by flow cytometry and presented as a percentage of the live cell population.

PROTOCOLS
Read this document before you begin with your experiment