BTXpress® electroporation buffer is a single buffer solution, developed to quickly and efficiently deliver genes into mammalian cells that were previously considered hard to transfect by chemical and other non-viral methods. This solution, in combination with BTX electroporators, provides researchers with the versatility needed for success across a broad range of cell types while maintaining critical cell viability. Transfection using this high performance electroporation solution is equally effective in delivering DNA as well as siRNA into mammalian cells.
The BTXpress solution offers increased numbers of transfections per kit compared to our competitors, providing better value to the researcher. As a universal solution, the BTXpress™ electroporation reagent can be used with competitor systems, including the Amaxa™ Nucleofector to deliver similar to equal performance without the cost associated with other systems. The BTXpress™ High Performance Electroporation Solution is offered as kits which includes the BTX Plus cuvettes with transfer pipettes or as a buffer alone.
- Efficient electroporation transfection
- High efficiency transfection of difficult cell lines
- High cell viability
- Low toxicity resulting in improved cell viability
- Single buffer for all cell types
- One buffer used in place of standard electroporation buffers for all mammalian cell types
Table 1: BTXpress® “High Performance Electroporation Solution" vs. Amaxa™ Nucleofector®. Cells were electroporated with an EGFP reporter vector in parallel, using the BTX ECM 830 Square Wave Electroporator with the BTXpress™ High Performance Electroporation Solution or using the Amaxa™ (Lonza) system. EGFP expressing cells were identified 24hrs post-electroporation by flow cytometry and presented as a percentage of the live cell population.
Table 2: BTXpress® Solution Transfection Efficiency vs. Amaxa™: Cells were electroporated in parallel with an EGFP reporter vector using either the BTX electroporation system with BTXpress™ High Performance Electroporation Solution or PBS. In comparison to the same cells transfected in the Amaxa™ (Lonza) system using the Amaxa™ kit V solution. The EGFP expressing cells were identified 24hrs post-electroporation by flow cytometry and presented as a percentage of the live cell population.
|Item #||Product||Included Items|
|45-0803||BTXpress Solution Kit 50 Reactions in 2 mm gap Cuvettes||5 mL bottle BTXpress solution and 5 bags (10 cuvettes/bag) of 2 mm gap BTXplus cuvettes with transfer pipette|
|45-0804||BTXpress Solution Kit 20 Reactions in 4 mm gap Cuvettes||5 mL bottle BTXpress solution and 2 bags of (10 cuvettes/bag) of 4mm gap BTXplus cuvettes with transfer pipette|
|45-0806||BTXpress Solution Kit 100 Reactions in 2 mm gap Cuvettes||10 mL bottle of BTXpress solution and 2 bags (50 cuvettes/bag) of 2 mm gap BTXplus cuvettes with transfer pipette|
|45-0807||BTXpress Solution Kit 40 Reactions in 4 mm gap Cuvettes||10 mL bottle of BTXpress solution and 4 bags (10 cuvettes/bag) of 4 mm gap BTXplus cuvettes with transfer pipette|
|45-0802||BTXpress Solution 5 mL||5 mL bottle for up to 50 reactions|
|45-0805||BTXpress Solution 10 mL||10 mL bottle for up to 100 reactions|
|Volume||2.0, 5.0, 10.0 ml|
|RNase||<0.5 pg of RNAse activity|
|DNase||<10 pg of DNAse activity|
Sterile filtered through 0.2 micron filter
|Storage||2 to 8°C|
|Shelf Life||1 year from date of purchase|