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BTXpress Electroporation Buffer

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BTXpress Electroporation Buffer
Single buffer solution, developed to quickly and efficiently deliver genes into mammalian cells that were previously considered “hard to transfect” by chemical and other non-viral methods.
Product Item # Price  
BTXpress Solution Kit, 50 Reactions in 2 mm Gap Cuvettes 45-0803   View Price
BTXpress Solution Kit, 20 Reactions in 4 mm Gap Cuvettes 45-0804   View Price
BTXpress Solution Kit, 100 Reactions in 2 mm Gap Cuvettes 45-0806   View Price
BTXpress Solution Kit, 40 Reactions in 4 mm Gap Cuvettes 45-0807   View Price
BTXpress Solution, 2.0 ml Bottle for up to 20 Reactions 45-0801   View Price
BTXpress Solution, 5.0 ml Bottle for up to 50 Reactions 45-0802   View Price
BTXpress Solution, 10.0 ml Bottle for up to 100 Reactions 45-0805   View Price
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Details

BTXpress® electroporation buffer is a single buffer solution, developed to quickly and efficiently deliver genes into mammalian cells that were previously considered hard to transfect by chemical and other non-viral methods. This solution, in combination with BTX electroporators, provides researchers with the versatility needed for success across a broad range of cell types while maintaining critical cell viability. Transfection using this high performance electroporation solution is equally effective in delivering DNA as well as siRNA into mammalian cells.

The BTXpress solution offers increased numbers of transfections per kit compared to our competitors, providing better value to the researcher. As a universal solution, the BTXpress™ electroporation reagent can be used with competitor systems, including the Amaxa™ Nucleofector to deliver similar to equal performance without the cost associated with other systems. The BTXpress™ High Performance Electroporation Solution is offered as kits which includes the BTX Plus cuvettes with transfer pipettes or as a buffer alone.

  • Efficient electroporation transfection
  • High efficiency transfection of difficult cell lines
  • High cell viability
  • Low toxicity resulting in improved cell viability
  • Single buffer for all cell types
  • One buffer used in place of standard electroporation buffers for all mammalian cell types

APPLICATIONS

 

Table 1: BTXpress® “High Performance Electroporation Solution" vs. Amaxa™ Nucleofector®. Cells were electroporated with an EGFP reporter vector in parallel, using the BTX ECM 830 Square Wave Electroporator with the BTXpress™ High Performance Electroporation Solution or using the Amaxa™ (Lonza) system. EGFP expressing cells were identified 24hrs post-electroporation by flow cytometry and presented as a percentage of the live cell population.

 

Table 2: BTXpress® Solution Transfection Efficiency vs. Amaxa™: Cells were electroporated in parallel with an EGFP reporter vector using either the BTX electroporation system with BTXpress™ High Performance Electroporation Solution or PBS. In comparison to the same cells transfected in the Amaxa™ (Lonza) system using the Amaxa™ kit V solution. The EGFP expressing cells were identified 24hrs post-electroporation by flow cytometry and presented as a percentage of the live cell population.

PROTOCOLS

 


INCLUDED ITEMS

Item # Product Included Items
45-0803 BTXpress Solution Kit 50 Reactions in 2 mm gap Cuvettes 5 mL bottle BTXpress solution and 5 bags (10 cuvettes/bag) of 2 mm gap BTXplus cuvettes with transfer pipette
45-0804 BTXpress Solution Kit 20 Reactions in 4 mm gap Cuvettes 5 mL bottle BTXpress solution and 2 bags of (10 cuvettes/bag) of 4mm gap BTXplus cuvettes with transfer pipette
45-0806 BTXpress Solution Kit 100 Reactions in 2 mm gap Cuvettes 10 mL bottle of BTXpress solution and 2 bags (50 cuvettes/bag) of 2 mm gap BTXplus cuvettes with transfer pipette
45-0807 BTXpress Solution Kit 40 Reactions in 4 mm gap Cuvettes 10 mL bottle of BTXpress solution and 4 bags (10 cuvettes/bag) of 4 mm gap BTXplus cuvettes with transfer pipette
45-0802 BTXpress Solution 5 mL 5 mL bottle for up to 50 reactions
45-0805 BTXpress Solution 10 mL 10 mL bottle for up to 100 reactions
Volume   2.0, 5.0, 10.0 ml 
Endotoxin  <0.125 EU/ml
 RNase  <0.5 pg of RNAse activity
 DNase  <10 pg of DNAse activity
 Sterility

Sterile filtered through 0.2 micron filter
Assayed for biological contamination for a minimum of 3 days  

 Storage  2 to 8°C
 Shelf Life 1 year from date of purchase
  • Transfection