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AgilePulse Needle Array Electrodes

  • Image 1
  • Needle Array handle
  • 6 mm gap, 10 mm length, 6 Needle Array (47-0070)



AgilePulse Needle Array Electrodes are miniature needles designed to provide superior, highly-uniform electric fields in dermal or muscular tissue as part of the AgilePulse™ in vivo Gene Delivery Electroporation System. Applications for these electrodes include intra-dermal DNA vaccine research and antibody production, cancer chemotherapy research or intra-muscular gene therapy. The miniature parallel-needle array is inserted directly into the target site for fast, reliable in vivo electroporation. The electric fields produced are the closest approximation of parallel plate electrodes, treating approximately 80% of the target area with 95% of the applied electric field. The Needle Array requires the Needle Array handle which includes the Electrode Connector cable. Several needle lengths and row spacing are available for various intra-dermal or intra-muscular applications. Needle Arrays can be used for up to 500 pulses with proper care and maintenance.

Applications

  • DNA Vaccine Delivery
  • Cancer Vaccine Research
  • Intra-dermal Transfection
  • Gene Therapy Research
  • Intra-muscular Gene Delivery
  • Chemotherapeutic Research

 

NEEDLE ARRAY DESIGN
  • Uniform, Reliable Electric Fields
  • Miniature Needles Minimize Tissue Trauma
  • Disposable, Sterile
  • Medical-grade plastic and surgical steel construction
  • Safety-assured design
  • Multiple configurations and sizes to choose
needlefields.jpg

Use of the AgilePulse HT system (formerly Cyto LVT-P) is subject to an annual license agreement between Cellectis bioresearch and the purchaser. Please contact Cellectis bioresearch cytopulse@cellectis.com for further details.

Use of the Hybrimune® device is subject to an annual license agreement between Cellectis bioresearch and the purchaser. Please contact Cellectis bioresearch cytopulse@cellectis.com for further details.

Use of the Agile Pulse In Vivo system (formerly the DermaVax device) is not authorized for any veterinary and human therapeutic uses. Contact Cellectis therapeutics contact@cellectis-therapeutics.com for such uses.



Needle Specifications for Intra Dermal ID SPECIFICATIONS
  47-0040 47-0043 47-0045 47-0050 47-0060
Needle Material Stainless Steel Stainless Steel Stainless Steel Stainless Steel Stainless Steel
Needle Tip Fine Point Fine Point Fine Point Fine Point Fine Point
Spacing in Row 1.5 mm 1.5 mm 1.5 mm 1.5 mm 1.5 mm
Spacing Between Rows 4 mm 4 mm 4 mm 4 mm 6 mm
Needles per Row 4 3 4 6 6
Needle Diameter 0.3 mm 0.3 mm 0.3 mm 0.3 mm 0.3 mm
Needle Length 2 mm 3 mm 5 mm 2 mm 5 mm
       
needlespecs.jpg
47-0070 Intra Muscular        47-0080 Large Intra Muscular   
       
LARGE NEEDLE ARRAY Intra Muscular Specification
  47-0070  47-0080  47-0086 
Needle Material  Stainless Steel  Stainless Steel  Stainless Steel 
Needle Tip  Fine Point  Trocar Point  Trocar Point 
Spacing in Row 1.5 mm 1.5 mm 1.5 mm
Spacing Between Rows 6 mm 6 mm 6 mm
Needles per Row 6 6 6
Needle Diameter 0.3 mm 0.7 mm 0.7 mm
Needle Length 10 mm 25 mm 16 mm
Cat. No Site Space between Rows Needles per Row Needle Length
47-0040  Intra Dermal  4 mm 4 2 mm 
47-0043  Intra Dermal  4 mm 3 3 mm 
47-0045  Intra Dermal  4 mm 4 5 mm 
47-0050 Intra Dermal 4 mm 6 2 mm
47-0060 Intra Dermal 6 mm 6 2 mm
47-0070 Intra Muscular 6 mm 6 10 mm
47-0080 Intra Muscular 6 mm 6 25 mm
47-0086 Intra Muscular 6 mm 6 16 mm
         
Cat No. Description Qty For Use With
47-0000 Needle Array Handle 1EA Fits all parallel needle arrays
       

Electrode Tips are shipped in individual Tyvek pouches. A cover is included to protect the needles during shipping and handling. Side grips provide on the body ensure safe handling and easy application.

BTX Protocol Database

BTX Monthly Tech Trends Articles

The BTX Current

In Vivo Tutorials

Technical Specifications

User's Manual

 

For further references regarding specific applications and optimization, please contact BTX Technical Support:

 

BTX-Division of Harvard Apparatus

84 October Hill Road 

Holliston, MA 01746 

Phone: 1-508-893-8999

Toll Free: 1-800-272-2775

Fax: 1-508-429-5732

Email: techsupport.btx@harvardapparatus.com

 

If outside the United States or Canada: call 508-893-8999 or

contact your nearest BTX Distributor

Lundberg K, Roos A-K, Pavlenko M, Wehrum D, Pisa P. Peptide specificity of HLA-A2-restricted CD8+ T cell responses induced with DNA vaccines coding for human and rhesus PSA. Vaccine. 2009 Mar 4;27(10):1557-65. Epub 2009 Jan 24

Bråve A, Hallengärd D, Gudmundsdotter L, Stout R, Walters R, Wahren B, Hallermalm K. Late administration of plasmid DNA by intradermal electroporation efficiently boosts DNA-primed T and B cell responses to carcinoembryonic antigen. Vaccine. 2009 Jun 8;27(28):3692-6. Epub 2009 May 3.

Roos, A-K, Eriksson, F, Walters, D, Pisa, P, King, A. Optimization of skin electroporation in mice to increase tolerability of DNA vaccine delivery to patients. Molecular Therapy, 2009 Sep;17(9):1637-42. Epub 2009 Jun 16.

Lladser A, Ljungberg K, Tufvesson H, Tazzari M, Roos A, Quest FG, Kiessling R. Intradermal electroporation with a survivin DNA vaccine induces CTLs against a self-epitope, suppresses angiogenesis and confers long-term protection against mouse


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