APPLICATIONS

• DNA Transfection
• RNA Transfection
• siRNA Transfection
• Cell Loading
• Mammalian Cell Transfection
  

EFFICIENT TRANSFECTION FOR UP TO 20 mL VOLUMES

The AgilePulse MAX^™ System is an advanced electroporation solution for fast, efficient transfection of 2 to 20 mL of cell suspension. Specifically engineered for large-volume applications, our system maximizes cellular uptake with minimal heating and short cycle-time to ensure high cell viability in further cell processing.

Simple-to-use, cells and polynucleotide are suspended in our proprietary BTXpress Cytoporation^® Medium T and transferred via sterile syringe to the large volume electroporation chamber where a programmed sequence of electric pulses is applied. First, a sequence of short, high-intensity pulses opens pores in the cell membranes, followed by long, low-intensity pulses that drive the material into cells via electrophoresis. Our patented PulseAgile^® technology optimizes these pulse parameters to maximize efficiency and cell viability. The system includes a user-friendly, programmable waveform generator with patented PulseAgile^® technology, our patent-pending large-volume electroporation chamber, and proprietary BTXpress Cytoporation^® Medium T, optimized for large volume electroporation. The system is engineered to provide uniform electric fields in a stable temperature environment for excellent cell viability.

LARGE-VOLUME ELECTROPORATION APPLICATIONS

• Transfect cells such as bone marrow cells to produce or replace a missing protein
• Deliver siRNA to suppress gene expression
• Deliver genes for permanent gene correction
• Load cells with a drug for drug delivery
• Cancer immunotherapy
• Transfect eukaryotic cells for protein production in bioreactors
• Large-scale production of replication-deficient viruses

POTENTIAL LICENSE REQUIREMENTS

No license is required for research use of the AgilePulse MAX^™ System but may be required for clinical and therapeutic use. Please contact BTX for more information.

Use of the AgilePulse HT system (formerly Cyto LVT-P) is subject to an annual license agreement between Cellectis bioresearch and the purchaser. Please contact Cellectis bioresearch cytopulse@cellectis.com for further details.

Use of the Hybrimune^® device is subject to an annual license agreement between Cellectis bioresearch and the purchaser. Please contact Cellectis bioresearch cytopulse@cellectis.com for further details.

Use of the Agile Pulse In Vivo system (formerly the DermaVax device) is not authorized for any veterinary and human therapeutic uses. Contact Cellectis therapeutics contact@cellectis-therapeutics.com for such uses.