APPLICATIONS

• DNA Transfection
• RNA Transfection
• siRNA Transfection
• Cell Loading
• Mammalian Cell Transfection
  

The AgilePulse HT™ system is an advanced electroporation solution for fast, efficient transfection of High Throughput Volumes, 50 mL to 1000 mL of cell suspension. Specifically engineered for high throughput volume applications, the AgilePulse HT™ system uses automated batch processing of smaller volumes to ensure uniform electroporation with minimal sample heating for high efficiency and excellent cell viability. AgilePulse HT™ is a bench-top system with two independent input pumps that simultaneously transport small-batch volumes of cell and transfectant solutions to the electroporation chamber for static electroporation. A third output pump then transfers the transfected cells to the collection site. Another batch of cells and transfectant are automatically transferred into the electroporation chamber, repeating the cycle 10 to 200 times until the entire volume of cells has been transfected. Each cycle takes only 7 seconds for fast, efficient transfection of very-large volumes of cells. The system includes a user-friendly, programmable waveform generator with patented Pulse Agile^® technology, our patent-pending large-volume electroporation chamber, and proprietary Cytoporation^® medium, optimized for large-volume electroporation.

LARGE-VOLUME ELECTROPORATION APPLICATIONS

• Transfect cells such as bone marrow cells to produce or replace a missing protein
• Deliver siRNA to suppress gene expression
• Deliver genes for permanent gene correction
• Load cells with a drug for drug delivery
• Cancer immunotherapy
• Transfect eukaryotic cells for protein production in bioreactors
• Large-scale production of replication-deficient viruses

AgilePulse HT is an automated system that can process up to 1 L of cell suspension in 5 mL static increments. One complete cycle takes 7 seconds. This system simultaneously pumps a cell suspension and polynucleotide solution into the electroporation chamber. The complex electroporation waveform is applied. The transfected solution is them pumped out of the chamber. This cycle is repeated until the complete volume has been processed. The system is sterile and closed.

Use of the AgilePulse HT system (formerly Cyto LVT-P) is subject to an annual license agreement between Cellectis bioresearch and the purchaser. Please contact Cellectis bioresearch cytopulse@cellectis.com for further details.

Use of the Hybrimune^® device is subject to an annual license agreement between Cellectis bioresearch and the purchaser. Please contact Cellectis bioresearch cytopulse@cellectis.com for further details.

Use of the Agile Pulse In Vivo system (formerly the DermaVax device) is not authorized for any veterinary and human therapeutic uses. Contact Cellectis therapeutics contact@cellectis-therapeutics.com for such uses.