The Adherent Cell Electrode is a reusable electroporation applicator designed to fit into each single well of a 12-well plate, 6-well plate or an individual 35 mm diameter Petri dish. This plate electrode is specially designed to electroporate cultured monolayers of cells on laminin-coated filters, or on 12 mm polycarbonate or polyester transwell filter units (0.4 mm pore size) or can directly electroporate adherent cells such as human corneal keratocytes, Caco-2, dental pulp cells, NC65 and BCEC and MDCK cells.  The Adherent Cell Electrode consists of two parallel plate electrodes, 7 x 19 mm with 5 mm gap spacing between the plates. Each plate electrode has a 0.3 mm height insulated foot at both ends of the bottom. When the electrode is placed in a dish, a foot minimizes the damage on cells. The high voltage cables are connected to a BTX electroporator, and the electrode is brought down onto the filter until it makes contact with the buffer, usually at 1- 2 mm above the filter. A combination of HV and LV pulses may be used. 

APPLICATIONS

• Mammalian Cell Transfections
• Direct electroporation onto adherent cells either growing on transwell filters or coverslips, or directly onto monolayers (such as polarized epithelial) without disruption
• Plant/Yeast Applications