The BTX 2-Needle Array Electrode is an in vivo style electrode specifically designed for muscle gene delivery. The electrode consists of a reusable Delrin 2-needle array handle and disposable two-needle array assemblies. The needle array assemblies are conveniently packaged in "six packs." The needles themselves are made of medical grade stainless steel.
Grasping the Needle Array handle, simply position the handle over a needle array assembly and push to secure the needle array onto the handle. Attach the handle to a BTX pulse generator via the high voltage banana cables. Remove the needle safety shield, place into the tissue, and deliver the pulse. Discard the needle array and prepare for the next experiment.
The 2-Needle Array is available in 5 mm and 10 mm electrode gap (distance between electrodes). The 45-0167 Handle is designed for the 45-0120 10 mm needle tips and used for larger muscle masses, such as rat gastrocnemius. The 45-0168 Handle is designed for the 45-0121 5 mm needle tips and is recommended for smaller muscle masses, such as mouse tibialis. Other species and tissues may be electroporated with the 2-Needle Array.
Among the non-viral techniques for gene transfer in vivo, the direct injection of plasmid DNA into muscle is simple, inexpensive, and safe. In vivo gene delivery by injection and electroporation of DNA into muscle tissue has been shown to enhanced gene expression by 100 fold compared to injection alone. DNA vaccination by direct in vivo administration of plasmid-based DNA vectors has proven to be very effective in animal models. It has been demonstrated in the literature that non-viral electroporation enhances gene expression in muscle greatly, making it possible to induce immune response in large animals. Therefore, in vivo electroporation is proven to be especially useful at increasing effectiveness of DNA vaccinations in large animals.
USE OF 2-NEEDLE ARRAY ELECTRODES WITH THE ECM 2001 ELECTRO CELL FUSION & ELECTROPORATION SYSTEM
In this example, the ECM 2001 generator is connected to the electrodes using the sequence of cables and adapters illustrated below, then connected to the array electrode. These electrodes are also compatible with the ECM 830 and Gemini X2. Using the ECM 830, all accessories plug directly into the unit.
|ECM 2001 System||45-0083 Coaxial Cables||45-0088 Female/Female Adapters|
USE OF 2-NEEDLE ARRAY ELECTRODES WITH THE GEMINI X2 ELECTROPORATION SYSTEM
In this example, the Gemini X2 is connected to the electrodes using the sequence of cables and adapters illustrated below, then connected to the array electrode.
|Gemini X2 System||45-0217 Flat Electrode Cables||45-0088 Female/Female Adapters|
|Voltage Range||0 - 500 V|
|Pulse Length Range:||1 µsec - 99 msec in PBS|
|Handle Length||8 cm||8 cm|
|Electrode Gap||10 mm||5 mm|
|2-Needle Array Assemblies||45-1020||45-1021|
|Needle Length||20 mm||20 mm|
|Needle Material||Stainless Steel||Stainless Steel|
|Electrode Gap||10 mm||5 mm|
|Generator Compatibility||Gemini X2, ECM 830 and ECM 2001|
- In Vivo Drug or Gene Delivery
- Intra Muscular Gene Therapy
- Intra Dermal Gene Therapy
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2. Nakano et al., Human Gene Therapy, 12: 1289-1297 (2001).
3. Widera et al., The Journal of Immunology, 164: 4635-4640 (2000).
4. Mir et al., PNAS, 96: 4262-4267 (1999).
5. Aihara and Miyazaki, Nature Biotechnology, 16: 867-870 (1998).